canine chondrocyte differentiation medium  (Cell Applications Inc)

Journal: Regenerative Therapy

Article Title: Generation of canine induced pluripotent stem cell-derived mesenchymal stem cells: Comparison of differentiation strategies and cell origins

doi: 10.1016/j.reth.2025.05.008

Figure Lengend Snippet: Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).

Article Snippet: The cells were then cultured in canine chondrocyte differentiation medium (Cell Applications) for 30 days at 37 °C and 5 % CO 2 .

Techniques: Derivative Assay, Staining, Quantitative Proteomics, Standard Deviation, Marker

Journal: Genes

Article Title: Expression Profile of New Gene Markers Involved in Differentiation of Canine Adipose-Derived Stem Cells into Chondrocytes

doi: 10.3390/genes13091664

Figure Lengend Snippet: The results of alcian blue staining of chondrocyte and control spheroid slides. The pictures were taken at a 10× magnification.

Article Snippet: For differentiation, the medium in the 96-well U-bottom plates containing ASC spheroids was exchanged to a commercially sourced chondrocyte differentiation medium (CN411D-250, Cell Applications, Inc., San Diego, CA, USA).

Techniques: Staining, Control

Journal: Genes

Article Title: Expression Profile of New Gene Markers Involved in Differentiation of Canine Adipose-Derived Stem Cells into Chondrocytes

doi: 10.3390/genes13091664

Figure Lengend Snippet: Differentially expressed gene of interest list between differentiated chondrocytes and day 30 culture control. FC—fold change, adj. p value—adjusted p value.

Article Snippet: For differentiation, the medium in the 96-well U-bottom plates containing ASC spheroids was exchanged to a commercially sourced chondrocyte differentiation medium (CN411D-250, Cell Applications, Inc., San Diego, CA, USA).

Techniques: Control

Journal: Genes

Article Title: Expression Profile of New Gene Markers Involved in Differentiation of Canine Adipose-Derived Stem Cells into Chondrocytes

doi: 10.3390/genes13091664

Figure Lengend Snippet: Heatmap presenting the changes in the 10 most up- and down-regulated genes between differentiated chondrocytes and day 1 primary ASC culture (early control), presented as log2FC.

Article Snippet: For differentiation, the medium in the 96-well U-bottom plates containing ASC spheroids was exchanged to a commercially sourced chondrocyte differentiation medium (CN411D-250, Cell Applications, Inc., San Diego, CA, USA).

Techniques: Control

Journal: Genes

Article Title: Expression Profile of New Gene Markers Involved in Differentiation of Canine Adipose-Derived Stem Cells into Chondrocytes

doi: 10.3390/genes13091664

Figure Lengend Snippet: Heatmap presenting the changes in the 10 most up- and down-regulated genes between differentiated chondrocytes and day 30 primary cASC culture (late control), presented as log2FC.

Article Snippet: For differentiation, the medium in the 96-well U-bottom plates containing ASC spheroids was exchanged to a commercially sourced chondrocyte differentiation medium (CN411D-250, Cell Applications, Inc., San Diego, CA, USA).

Techniques: Control